Tested Applications
| Positive WB detected in | human skeletal muscle tissue, mouse skeletal muscle tissue, mouse testis tissue, rat skeletal muscle tissue, mouse eye tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
88228-1-RR targets TMEM219 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg10278 Product name: Recombinant human TMEM219 protein Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 39-204 aa of NM_001083613.2 Sequence: SFLLTHRTGLRSPDIPQDWVSFLRSFGQLTLCPRNGTVTGKWRGSHVVGLLTTLNFGDGPDRNKTRTFQATVLGSQMGLKGSSAGQLVLITARVTTERTAGTCLYFSAVPGILPSSQPPISCSEEGAGNATLSPRMGEECVSVWSHEGLVLTKLLTSEELALCGSR Predict reactive species |
| Full Name | transmembrane protein 219 |
| Calculated Molecular Weight | 26 kDa |
| Observed Molecular Weight | 26~30 kDa |
| GenBank Accession Number | NM_001083613.2 |
| Gene Symbol | TMEM219 |
| Gene ID (NCBI) | 124446 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q86XT9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TMEM219, also known as IGFBP-3R (Insulin-like growth factor-binding protein 3 receptor), is a recently discovered death receptor, which induces cell apoptosis by a Caspase-8-dependent mechanism, through the binding with its ligand, the Insulin-like growth factors binding protein 3 (IGFBP3). TMEM219 was one of the most highly expressed and ranked the third most abundant beta-cell receptor. Increased TMEM219 expression was primarily evident in intestinal cells cultured with inflammatory cytokines and it was associated with higher cell death
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TMEM219 antibody 88228-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
