Home
Products
Primary Antibodies
Recombinant Antibodies
Cleaved GSDME (Asp270), C-terminal Recombinant monoclonal antibody (88249-1-RR)

Cleaved GSDME (Asp270), C-terminal Recombinant monoclonal antibody

Cleaved GSDME (Asp270), C-terminal Uni-rAb^® Recombinant Antibody for WB, FC (Intra), ELISA

Cat No. 88249-1-RR

Clone No.253373H3

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

Cleaved Gasdermin E, cleaved GSDME, cleaved GSDME (Asp270), DFNA5, DFNA5/GSDME

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Filter: AllWBFC (Intra)

WB analysis of A431 using 88249-1-RR
Doxorubicin hydrochloride treated A431 cells were subjected to SDS PAGE followed by western blot with 88249-1-RR (Cleaved GSDME (Asp270), C-terminal antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of SH-SY5Y using 88249-1-RR
etoposide treated SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 88249-1-RR (Cleaved GSDME (Asp270), C-terminal antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of SH-SY5Y using 88249-1-RR
1X10^6 SH-SY5Y cells untreated (dashed lines) or treated with etoposide which intracellularly stained with 0.25 ug cleaved GSDME (Asp270), C-terminal Recombinant monoclonal antibody (88249-1-RR, Clone:253373H3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
1X10^6 SH-SY5Y cells untreated (dashed lines) or treated with etoposide which intracellularly stained with 0.25 ug cleaved GSDME (Asp270), C-terminal Recombinant monoclonal antibody (88249-1-RR, Clone:253373H3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

Overview

Tested Applications
Product Information
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	Doxorubicin hydrochloride treated A431 cells, etoposide treated SH-SY5Y cells
Positive FC (Intra) detected in	etoposide treated SH-SY5Y cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

88249-1-RR targets Cleaved GSDME (Asp270), C-terminal in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	deafness, autosomal dominant 5
Calculated Molecular Weight	496 aa, 55 kDa
Observed Molecular Weight	25 kDa
GenBank Accession Number	BC019689
Gene Symbol	DFNA5
Gene ID (NCBI)	1687
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	O60443
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GSDME, also known as DFNA5 (deafness, autosomal dominant 5), is a member of the gasdermin family of pore-forming proteins. In its full-length form, GSDME is autoinhibited. Upon apoptotic stimulation, active caspase-3 specifically cleaves GSDME at a conserved aspartate residue, Asp270 (D270). This cleavage of Gasdermin E separates the N‑terminal pore‑forming domain from the C‑terminal repressor domain. The released N‑terminal fragment then inserts into the plasma membrane, forms large pores, and induces a lytic, inflammatory form of cell death known as pyroptosis. Therefore, Asp270 cleavage is a critical molecular switch that converts caspase‑3‑mediated apoptosis into pyroptosis, linking GSDME/DFNA5 to cancer therapy, chemotherapy‑induced toxicity, and hearing loss pathology.

Protocols

Product Specific Protocols
FC protocol for Cleaved GSDME (Asp270), C-terminal antibody 88249-1-RR	Download protocol
WB protocol for Cleaved GSDME (Asp270), C-terminal antibody 88249-1-RR	Download protocol