Cleaved GSDME (Asp270), C-terminal Recombinant monoclonal antibody, PBS Only

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• Cleaved GSDME (Asp270), C-terminal Recombinant monoclonal antibody, PBS Only (88249-1-PBS)

Cleaved GSDME (Asp270), C-terminal Uni-rAb^® Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Cat No. 88249-1-PBS

Clone No.253373H3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

Cleaved Gasdermin E, cleaved GSDME, cleaved GSDME (Asp270), DFNA5, DFNA5/GSDME

• 

  WB analysis of A431 using 88249-1-RR (same clone as 88249-1-PBS)

  Doxorubicin hydrochloride treated A431 cells were subjected to SDS PAGE followed by western blot with 88249-1-RR (Cleaved GSDME (Asp270), C-terminal antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88249-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of SH-SY5Y using 88249-1-RR (same clone as 88249-1-PBS)

  etoposide treated SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 88249-1-RR (Cleaved GSDME (Asp270), C-terminal antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88249-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of SH-SY5Y using 88249-1-RR (same clone as 88249-1-PBS)

  1X10^6 SH-SY5Y cells untreated (dashed lines) or treated with etoposide which intracellularly stained with 0.25 ug cleaved GSDME (Asp270), C-terminal Recombinant monoclonal antibody (88249-1-RR, Clone:253373H3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). This data was developed using the same antibody clone with 88249-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

88249-1-PBS targets Cleaved GSDME (Asp270), C-terminal in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: deafness, autosomal dominant 5
• Calculated Molecular Weight: 496 aa, 55 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC019689
• Gene Symbol: DFNA5
• Gene ID (NCBI): 1687
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O60443
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

GSDME, also known as DFNA5 (deafness, autosomal dominant 5), is a member of the gasdermin family of pore-forming proteins. In its full-length form, GSDME is autoinhibited. Upon apoptotic stimulation, active caspase-3 specifically cleaves GSDME at a conserved aspartate residue, Asp270 (D270). This cleavage of Gasdermin E separates the N‑terminal pore‑forming domain from the C‑terminal repressor domain. The released N‑terminal fragment then inserts into the plasma membrane, forms large pores, and induces a lytic, inflammatory form of cell death known as pyroptosis. Therefore, Asp270 cleavage is a critical molecular switch that converts caspase‑3‑mediated apoptosis into pyroptosis, linking GSDME/DFNA5 to cancer therapy, chemotherapy‑induced toxicity, and hearing loss pathology.