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NOC2L Recombinant monoclonal antibody, PBS Only (Capture) (88267-1-PBS)

NOC2L Recombinant monoclonal antibody, PBS Only (Capture)

Name: NOC2L Recombinant monoclonal antibody, PBS Only (Capture), 100ug
Brand: Proteintech
SKU: 88267-1-PBS-100UG

NOC2L Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Cat No. 88267-1-PBS

Clone No. 260756G2

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

NIR, NOC2-like protein, Novel INHAT repressor, Nucleolar complex protein 2 homolog, Protein NOC2 homolog

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

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Filter: AllWBIHCIF/ICCCytometric bead array sample testCytometric bead arrayAffinity

WB analysis using 88267-1-RR (same clone as 88267-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 88267-1-RR (NOC2L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88267-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 88267-1-RR (same clone as 88267-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 88267-1-RR (NOC2L antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88267-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human placenta using 88267-1-RR (same clone as 88267-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 88267-1-RR (NOC2L antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 88267-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of U2OS using 88267-1-RR (same clone as 88267-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed U2OS cells using NOC2L antibody (88267-1-RR, Clone: 260756G2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 88267-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Cytometric bead array sample test of MP03442-1
The mean NOC2L concentration was determined to be 26.5 ng/mL in Raji cell extract based on a 1.0 mg/mL extract load, 26.8 ng/mL in HT-1080 cell extract based on a 1.0 mg/mL extract load, 20.3 ng/mL in U2OS cell extract based on a 1.0 mg/mL extract load.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Cytometric bead array standard curve of MP03442-1
Cytometric bead array standard curve of MP03442-1, NOC2L Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 88267-1-PBS. Detection antibody: 88267-2-PBS. Standard: Ag26952. Range: 0.156-20 ng/mL.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Affinity of 88267-1-RR
Biolayer interferometry (BLI) kinetic assays of 88267-1-RR against Human NOC2L were performed. The affinity constant is 0.413 nM.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information

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Product Information

88267-1-PBS targets NOC2L as part of a matched antibody pair:

MP03442-1: 88267-1-PBS capture and 88267-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag26952 Product name: Recombinant human NOC2L protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-202 aa of BC003555 Sequence: MAAAGSRKRRLAELTVDEFLASGFDSESESESENSPQAETREAREAARSPDKPGGSPSASRRKGRASEHKDQLSRLKDRDPEFYKFLQENDQSLLNFSDSDSSEEEEGPFHSLPDVLEEASEEEDGAEEGEDGDRVPRGLKGKKNSVPVTVAMVERWKQAAKQRLTPKLFHEVVQAFRAAVATTRGDQESAEANKFQVTDSA Predict reactive species
Full Name	nucleolar complex associated 2 homolog (S. cerevisiae)
Calculated Molecular Weight	749 aa, 85 kDa
Observed Molecular Weight	85 kDa
GenBank Accession Number	BC003555
Gene Symbol	NOC2L
Gene ID (NCBI)	26155
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q9Y3T9
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

NOC2L, also known as NIR, is a transcriptional corepressor with inhibitor of histone acetyltransferase (INHAT) activity (PMID: 16322561). It prevents acetylation of all core histones by the histone acetyltransferase EP300/p300. NOC2L interacts with p53, inhibits p53-activated gene expression, and regulates p53-dependent apoptosis (PMID: 16322561). NIR binds to TAp63 and acts as a repressor of TAp63-mediated transactivation of the p21Cip1 gene (PMID: 20123734). NOC2L is ubiquitously expressed throughout embryonic development and adulthood (PMID: 16322561). NOC2L has been reported as a key regulator of skin development. Epidermis-specific ablation of NOC2L impairs epidermal stratification and barrier function (PMID: 29490983).