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RAP1B Recombinant monoclonal antibody (88443-1-RR)

RAP1B Recombinant monoclonal antibody

RAP1B Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 88443-1-RR

Clone No.261087A12

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IF/ICC, ELISA

Synonyms

EC:3.6.5.2, GTP binding protein smg p21B, GTP-binding protein smg p21B, K REV, RAL1B

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

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Filter: AllWBIF/ICC

WB analysis using 88443-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 88443-1-RR (RAP1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of A549 using 88443-1-RR
Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using RAP1B antibody (88443-1-RR, Clone: 261087A12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, HEK-293 cells, HUVEC cells, COS-7 cells, NIH/3T3 cells, C6 cells
Positive IF/ICC detected in	A549 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

88443-1-RR targets RAP1B in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity	human, mouse, rat, monkey
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag1282 Product name: Recombinant human RAP1B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-184 aa of BC000176 Sequence: MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDAQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTDDVPMILVGNKCDLEDERVVGKEQGQNLARQWNNCAFLESSAKSKINVNEIFYDLVRQINRKTPVPGKARKKSSCQLL Predict reactive species
Full Name	RAP1B, member of RAS oncogene family
Calculated Molecular Weight	21 kDa
Observed Molecular Weight	21 kDa
GenBank Accession Number	BC000176
Gene Symbol	RAP1B
Gene ID (NCBI)	5908
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P61224
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAP1B is a member of the RAS-like small GTP-binding protein superfamily. Members of this family regulate multiple cellular processes including cell adhesion and growth and differentiation. RAP1B localizes to cellular membranes and has been shown to regulate integrin-mediated cell signaling. It also plays a role in regulating outside-in signaling in platelets.