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TCF4/TCF7L2 Recombinant monoclonal antibody, PBS Only (Capture) (89174-1-PBS)

TCF4/TCF7L2 Recombinant monoclonal antibody, PBS Only (Capture)

Name: TCF4/TCF7L2 Recombinant monoclonal antibody, PBS Only (Capture), 100ug
Brand: Proteintech
SKU: 89174-1-PBS-100UG

TCF4/TCF7L2 Uni-rAb^® Recombinant Antibody for WB, IHC, IP, Cytometric bead array, Indirect ELISA

Cat No. 89174-1-PBS

Clone No. 262241D1

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Cytometric bead array, Indirect ELISA

Synonyms

TCF7L2, HMG box transcription factor 4, hTCF 4, hTCF-4, T cell factor 4

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

SKU:

-/ -

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Quantity

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Filter: AllWBIPIHCCytometric bead array sample testCytometric bead arrayAffinity

WB analysis using 89174-1-RR (same clone as 89174-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 89174-1-RR (TCF7L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 89174-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of HEK-293 using 89174-1-RR (same clone as 89174-1-PBS)
IP result of anti-TCF4/TCF7L2 (IP:89174-1-RR, 4ug; Detection:89174-1-RR 1:2000) with HEK-293 cells lysate 1800 ug. This data was developed using the same antibody clone with 89174-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon using 89174-1-RR (same clone as 89174-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 89174-1-RR (TCF4/TCF7L2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 89174-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon using 89174-1-RR (same clone as 89174-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 89174-1-RR (TCF4/TCF7L2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 89174-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Cytometric bead array sample test of MP03819-1
The mean TCF4/TCF7L2 concentration was determined to be 162.3 ng/mL in Hela nuclear cell extract based on a 1.2 mg/mL extract load, 7.7 ng/mL in Hela cytoplasmic cell extract based on a 1.2 mg/mL extract load.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Cytometric bead array standard curve of MP03819-1
Cytometric bead array standard curve of MP03819-1, TCF4/TCF7L2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 89174-1-PBS. Detection antibody: 89174-2-PBS. Standard: Ag4729. Range: 1.25-80 ng/mL.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Affinity of 89174-1-RR
Biolayer interferometry (BLI) kinetic assays of 89174-1-RR against Human TCF4/TCF7L2 were performed. The affinity constant is 1.01 nM.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

89174-1-PBS targets TCF4/TCF7L2 as part of a matched antibody pair:

MP03819-1: 89174-1-PBS capture and 89174-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag4729 Product name: Recombinant human TCF7L2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-355 aa of BC032656 Sequence: MPQLNGGGGDDLGANDELISFKDEGEQEEKSSENSSAERDLADVKSSLVNESETNQNSSSDSEAERRPPPRSESFRDKSRESLEEAAKRQDGGLFKGPPYPGYPFIMIPDLTSPYLPNGSLSPTARTYLQMKWPLLDVQAGSLQSRQALKDARSPSPAHIVSNKVPVVQHPHHVHPLTPLITYSNEHFTPGNPPPHLPADVDPKTGIPRPPHPPDISPYYPLSPGTVGQIPHPLGWQGQPVYPITTGGFRHPYPTALTVNASMSRFPPHMVPPHHTLHTTGIPHPAIVTPTVKQESSQSDVGSLHSSKHQDSKKEEEKKKPHIKKPLNAFMLYMKEMRAKVVAECTLKESAAINQ Predict reactive species
Full Name	transcription factor 7-like 2 (T-cell specific, HMG-box)
Calculated Molecular Weight	619 aa, 68 kDa
Observed Molecular Weight	60, 75 kDa
GenBank Accession Number	BC032656
Gene Symbol	TCF4
Gene ID (NCBI)	6934
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q9NQB0
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

TCF7L2 encodes an HMG-box transcription factor and key effector of canonical Wnt/β-catenin signaling, regulating development and tissue homeostasis. It is the strongest genetic susceptibility locus for type 2 diabetes. Alternative splicing produces functionally distinct isoforms, and TCF7L2 variants have also been linked to Crohn's disease and cancer, indicating pleiotropic roles in disease.