Tested Applications
| Positive FC detected in | rat splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
98060-1-RR targets ICAM-1/CD54 in FC, Blocking assay applications and shows reactivity with rat samples.
| Tested Reactivity | rat |
| Cited Reactivity | rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg1025 Product name: Recombinant rat ICAM-1/CD54 protein Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Domain: 28-493 aa of NM_012967.1 Sequence: QVSIHPTEAFLPRGGSVQVNCSSSCEDENLGLGLETNWMKDELSSGHNWKLFKLSDIGEDSRPLCFENCGTTQSSASATITVYSFPERVELDPLPAWQQVGKNLILRCLVEGGAPRTQLSVVLLRGNETLSRQAVDGDPKEITFTVLASRGDHGANFSCFTELDLRPQGLSLFKNVSEVRQLRTFDLPTRVLKLDTPDLLEVGTQQKFLCSLEGLFPASEAQIYLEMGGQMLTLESTNSRDFVSATASVEVTEKLDRTLQLRCVLELADQTLEMEKTLRIYNFSAPILTLSQPEVSEGDQVTVKCEAHGGAQVVLLNSTSPRPPTSQGTSPRPPTSQIQFTLNASPEDHKRRFFCSAALEVDGKSLFKNQTLELHVLYGPHLDKKDCLGNWTWQEGSQQTLTCQPQGNPAPNLTCSRKADGVPLPIGMVKSVKREMNGTYKCRAFSSRGSITRDVHLTVLYHDQNT Predict reactive species |
| Full Name | intercellular adhesion molecule 1 |
| Calculated Molecular Weight | 60 kDa |
| GenBank Accession Number | NM_012967.1 |
| Gene Symbol | Icam1 |
| Gene ID (NCBI) | 25464 |
| RRID | AB_3672207 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q00238 |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2 - 8°C. Stable for one year after shipment. |
Background Information
ICAM-1 (CD54) is a transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for ICAM-1/CD54 antibody 98060-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Anti-Rat ICAM-1/CD54 Rabbit Recombinant Antibody (Cat# 98060-1-RR) has 1 citation to date. It was cited in ACS Applied Materials & Interfaces (2025), in a study on monocyte membrane-fused liposomes for targeted treatment of mesangioproliferative glomerulonephritis. The research falls within the fields of nanomedicine/drug delivery and kidney disease (glomerulonephritis), with the antibody used in a blocking assay on rat samples.
| Species | Application | Title |
|---|---|---|
ACS Appl Mater Interfaces Monocyte Membrane-Fused Liposomes for Enhanced Targeted Treatment of Mesangioproliferative Glomerulonephritis. |
