CoraLite® Plus 647 Anti-Rat ICAM-1/CD54 Rabbit Recombinant Antibody

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  FC experiment of rat splenocytes using CL647-98060

  1x10^6 rat splenocytes were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Rat ICAM-1/CD54 Rabbit RecAb (CL647-98060, Clone: 240725C2)(red) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). Cells were not fixed.

ICAM-1/CD54 Recombinant Antibody for FC

Cat No. CL647-98060

Clone No.240725C2

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

FC

Synonyms

CD54, ICAM-1, 240725C2, Icam1, ICAM-12

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Tested Applications

• Positive FC detected in: rat splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-98060 targets ICAM-1/CD54 in FC applications and shows reactivity with rat samples.

• Tested Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ICAM-1/CD54 fusion protein Eg1025
• Full Name: intercellular adhesion molecule 1
• Calculated Molecular Weight: 60 kDa
• GenBank Accession Number: NM_012967.1
• Gene Symbol: Icam1
• Gene ID (NCBI): 25464
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q00238
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

ICAM-1 (CD54) is a transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).