Tested Applications
| Positive FC detected in | human peripheral blood leukocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
98109-1-RR targets VISTA in FC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg0017 Product name: Recombinant Human VISTA/C10orf54 protein (Myc Tag, His Tag) Source: mammalian cells-derived, pHZ-KIsec Tag: Myc & 6*His Domain: 33-194 aa of BC020568 Sequence: FKVATPYSLYVCPEGQNVTLTCRLLGPVDKGHDVTFYKTWYRSSRGEVQTCSERRPIRNLTFQDLHLHHGGHQAANTSHDLAQRHGLESASDHHGNFSITMRNLTLLDSGLYCCLVVEIRHHHSEHRVHGAMELQVQTGKDAPSNCVVYPSSSQESENITAA Predict reactive species |
| Full Name | chromosome 10 open reading frame 54 |
| Calculated Molecular Weight | 311 aa, 34 kDa |
| GenBank Accession Number | BC020568 |
| Gene Symbol | VISTA |
| Gene ID (NCBI) | 64115 |
| RRID | AB_3672256 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q9H7M9 |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2 - 8°C. Stable for one year after shipment. |
Background Information
VISTA, also known as platelet receptor Gi24, B7-H5, C10orf54, Dies1, PD-1H, and SISP1, is a member of the immunoglobulin superfamily. It serves not only as a positive regulator but also as a substrate of MT1-MMP and contributes at least in part to tumor invasion.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for VISTA antibody 98109-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
