Anti-Human VISTA Rabbit Recombinant Antibody, PBS Only

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• Anti-Human VISTA Rabbit Recombinant Antibody, PBS Only (98109-1-PBS)

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  FC experiment of human peripheral blood leukocytes using 98109-1-RR (same clone as 98109-1-PBS)

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human VISTA Rabbit RecAb (98109-1-RR, Clone: 241003C8) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98109-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human peripheral blood leukocytes using 98109-1-RR (same clone as 98109-1-PBS)

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human VISTA Rabbit RecAb (98109-1-RR, Clone: 241003C8) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9)(blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98109-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98109-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98109-1-RR against Human VISTA were performed. The affinity constant is 0.933 nM.

VISTA Uni-rAb^® Recombinant Antibody for FC

Cat No. 98109-1-PBS

Clone No.241003C8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

C10orf54, 241003C8, B7-H5, GI24, PP2135

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Product Information

98109-1-PBS targets VISTA in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg0017

  Product name: Recombinant Human VISTA/C10orf54 protein (Myc Tag, His Tag)

  Source: mammalian cells-derived, pHZ-KIsec

  Tag: Myc & 6*His

  Domain: 33-194 aa of BC020568

  Sequence: FKVATPYSLYVCPEGQNVTLTCRLLGPVDKGHDVTFYKTWYRSSRGEVQTCSERRPIRNLTFQDLHLHHGGHQAANTSHDLAQRHGLESASDHHGNFSITMRNLTLLDSGLYCCLVVEIRHHHSEHRVHGAMELQVQTGKDAPSNCVVYPSSSQESENITAA

• Full Name: chromosome 10 open reading frame 54
• Calculated Molecular Weight: 311 aa, 34 kDa
• GenBank Accession Number: BC020568
• Gene Symbol: VISTA
• Gene ID (NCBI): 64115
• RRID: AB_3672256
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9H7M9
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

VISTA, also known as platelet receptor Gi24, B7-H5, C10orf54, Dies1, PD-1H, and SISP1, is a member of the immunoglobulin superfamily. It serves not only as a positive regulator but also as a substrate of MT1-MMP and contributes at least in part to tumor invasion.