Tested Applications
| Positive FC detected in | bEnd.3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
98251-1-RR targets VE-cadherin/CD144 in IF, FC applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Recombinant protein Predict reactive species |
| Full Name | cadherin 5 |
| Calculated Molecular Weight | 88kDa |
| GenBank Accession Number | NM_009868.4 |
| Gene Symbol | Cdh5 |
| Gene ID (NCBI) | 12562 |
| RRID | AB_3745918 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P55284 |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2 - 8°C. Stable for one year after shipment. |
Background Information
Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. Vascular endothelial cadherin (VE-cadherin), also known as Cadherin-5 (CDH5) or CD144, is a member of the type II classical cadherin family of cell adhesion proteins (PMID: 21269602). VE-cadherin is expressed specifically in endothelial cells and mediates homophilic adhesion in the vascular endothelium (PMID: 1522121; 8555485; 21269602). VE-cadherin plays a role in the organization of lateral endothelial junctions and in the control of permeability properties of vascular endothelium (PMID: 1522121). VE-cadherin has also been shown to be required for angiogenesis (PMID: 16473763; 18162609).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for VE-cadherin/CD144 antibody 98251-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Anti-Mouse VE-cadherin/CD144 Rabbit Recombinant Antibody (catalog 98251-1-RR) has 1 citation. It appears in BMC Pulm Med (2026), used for immunofluorescence in mouse. The research field is pulmonary medicine, specifically endothelial GABA(A) receptor α1 subunit activation protecting against barrier dysfunction in acute lung injury.
| Species | Application | Title |
|---|---|---|
BMC Pulm Med Activation of endothelial GABA(A) receptor α1 subunit protects against barrier dysfunction in acute lung injury. |
