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Anti-Mouse JAM3 Rabbit Recombinant Antibody (98393-2-RR)

Anti-Mouse JAM3 Rabbit Recombinant Antibody

JAM3 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98393-2-RR

Clone No. 242630E6

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Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

Synonyms

JAM-2, JAM-3, JAM-C, Junctional adhesion molecule 3, Junctional adhesion molecule C

Formulation: PBS, Azide

PBS, Azide

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

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FC experiment of Transfected HEK-293T using 98393-2-RR
1x10^6 HEK-293T cells or JAM3 Transfected HEK-293T cells were surface stained with 0.25 ug Anti-Mouse JAM3 Rabbit RecAb (98393-2-RR, Clone: 242630E6) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of Transfected HEK-293T using 98393-2-RR
1x10^6 HEK-293T cells or JAM3 Transfected HEK-293T cells were surface stained with 0.25 ug Anti-Mouse JAM3 Rabbit RecAb (98393-2-RR, Clone: 242630E6) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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What's the best dilution for JAM3 in ?

Overview

Tested Applications
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MSDS SDS

Tested Applications

Positive FC detected in

Transfected HEK-293T cells

Recommended dilution

Application	Dilution
Flow Cytometry (FC)	FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98393-2-RR targets JAM3 in FC applications and shows reactivity with mouse samples.

Tested Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg2661 Product name: Recombinant Mouse JAM-3 protein (rFc Tag)(HPLC verified) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 30-241 aa of NM_023277.4 Sequence: EAVNLKSSNRNPVVHEFESVELSCIITDSQTSDPRIEWKKIQDGQTTYVYFDNKIQGDLAGRTDVFGKTSLRIWNVTRSDSAIYRCEVVALNDRKEVDEITIELIVQVKPVTPVCRIPAAVPVGKTATLQCQESEGYPRPHYSWYRNDVPLPTDSRANPRFQNSSFHVNSETGTLVFNAVHKDDSGQYYCIASNDAGAARCEGQDMEVYDLN Predict reactive species
Full Name	junction adhesion molecule 3
Calculated Molecular Weight	35 kDa
GenBank Accession Number	NM_023277.4
Gene Symbol	Jam3
Gene ID (NCBI)	83964
RRID	AB_3746038
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q9D8B7
Storage Buffer	PBS with 0.09% sodium azide, pH 7.3.
Storage Conditions	Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Junctional adhesion molecule 3 (JAM3) is a type I transmembrane glycoprotein containing two Ig-like domains, which is expressed in various tissues and plays a crucial role in cell junctions, cell polarity, and motility (PMID: 34292449; 28931565). It has been proposed that JAM3 participates in leukocyte-platelet interactions, as well as angiogenesis and brain development (PMID: 12208882; 23255084). JAM3 may also be a new marker for predicting the prognosis and immune functions of bladder cancer patients (PMID: 38400838).