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Anti-Mouse TIM4 Rabbit Recombinant Antibody (98401-1-RR)

Anti-Mouse TIM4 Rabbit Recombinant Antibody

TIM4 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98401-1-RR

Clone No. 242616C10

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Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

Synonyms

SMUCKLER, Spleen, mucin-containing, knockout of lymphotoxin protein, T-cell immunoglobulin and mucin domain-containing protein 4, T-cell immunoglobulin mucin receptor 4, T-cell membrane protein 4

Formulation: PBS, Azide

PBS, Azide

PBS Only

Conjugate: Unconjugated

Unconjugated

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FC experiment of mouse peritoneal macrophages using 98401-1-RR
1x10^6 mouse peritoneal macrophages were surface stained with 0.25 ug Anti-Mouse TIM4 Rabbit RecAb (98401-1-RR, Clone: 242616C10) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-conjugated Goat Anti-Rabbit IgG. Cells were co-stained with CoraLite® Plus 647 Anti-Mouse F4/80. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of mouse peritoneal macrophages using 98401-1-RR
1x10^6 mouse peritoneal macrophages were surface stained with 0.25 ug Anti-Mouse TIM4 Rabbit RecAb (98401-1-RR, Clone: 242616C10) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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What's the best dilution for TIM4 in ?

Overview

Tested Applications
Product Information
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MSDS SDS

Tested Applications

Positive FC detected in

mouse peritoneal macrophages

Recommended dilution

Application	Dilution
Flow Cytometry (FC)	FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98401-1-RR targets TIM4 in FC applications and shows reactivity with mouse samples.

Tested Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg3307 Product name: Recombinant Mouse TIM4 protein (rFc Tag) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 23-279 aa of NM_178759 Sequence: ASEDTIIGFLGQPVTLPCHYLSWSQSRNSMCWGKGSCPNSKCNAELLRTDGTRIISRKSTKYTLLGKVQFGEVSLTISNTNRGDSGVYCCRIEVPGWFNDVKKNVRLELRRATTTKKPTTTTRPTTTPYVTTTTPELLPTTVMTTSVLPTTTPPQTLATTAFSTAVTTCPSTTPGSFSQETTKGSAFTTESETLPASNHSQRSMMTISTDIAVLRPTGSNPGILPSTSQLTTQKTTLTTSESLQKTTKSHQINSRQT Predict reactive species
Full Name	T-cell immunoglobulin and mucin domain containing 4
Calculated Molecular Weight	38 kDa
GenBank Accession Number	NM_178759
Gene Symbol	TIM4
Gene ID (NCBI)	276891
RRID	AB_3746044
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q6U7R4
Storage Buffer	PBS with 0.09% sodium azide, pH 7.3.
Storage Conditions	Store at 2 - 8°C. Stable for one year after shipment.

Background Information

T cell immunoglobulin mucin 4 (TIM4) is a macrophage receptor for phosphatidylserine (PtdSer) on apoptotic cells. It is evidenced that TIM4 as phosphatidylserine (PS) receptor is capable of maintaining peritoneal macrophages' homeostatic function and regulating adaptive immunity based on the alteration of Ag-particular T cells' clearance. It is noteworthy that TIM4's dysregulating process was identified under several autoimmune states, in particular inside immune cells. In addition, TIM4 was identified to be over-expressed in Systemic lupus erythematosus (SLE) cases.