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Anti-Mouse TIM4 Rabbit Recombinant Antibody, PBS Only (98401-1-PBS)

Anti-Mouse TIM4 Rabbit Recombinant Antibody, PBS Only

Name: Anti-Mouse TIM4 Rabbit Recombinant Antibody, PBS Only, 1mg
Brand: Proteintech
SKU: 98401-1-PBS-1MG

TIM4 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98401-1-PBS

Clone No. 242616C10

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Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

Synonyms

SMUCKLER, Spleen, mucin-containing, knockout of lymphotoxin protein, T-cell immunoglobulin and mucin domain-containing protein 4, T-cell immunoglobulin mucin receptor 4, T-cell membrane protein 4

Formulation: PBS Only

PBS, Azide

PBS Only

Conjugate: Unconjugated

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Filter: AllFC

FC experiment of mouse peritoneal macrophages using 98401-1-RR (same clone as 98401-1-PBS)
1x10^6 mouse peritoneal macrophages were surface stained with 0.25 ug Anti-Mouse TIM4 Rabbit RecAb (98401-1-RR, Clone: 242616C10) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-conjugated Goat Anti-Rabbit IgG. Cells were co-stained with CoraLite® Plus 647 Anti-Mouse F4/80. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98401-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
1x10^6 mouse peritoneal macrophages were surface stained with 0.25 ug Anti-Mouse TIM4 Rabbit RecAb (98401-1-RR, Clone: 242616C10) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-conjugated Goat Anti-Rabbit IgG. Cells were co-stained with CoraLite® Plus 647 Anti-Mouse F4/80. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98401-1-PBS in a different storage buffer formulation.
FC experiment of mouse peritoneal macrophages using 98401-1-RR (same clone as 98401-1-PBS)
1x10^6 mouse peritoneal macrophages were surface stained with 0.25 ug Anti-Mouse TIM4 Rabbit RecAb (98401-1-RR, Clone: 242616C10) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98401-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
1x10^6 mouse peritoneal macrophages were surface stained with 0.25 ug Anti-Mouse TIM4 Rabbit RecAb (98401-1-RR, Clone: 242616C10) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98401-1-PBS in a different storage buffer formulation.

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Tested Applications
Product Information

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Product Information

98401-1-PBS targets TIM4 in FC applications and shows reactivity with mouse samples.

Tested Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg3307 Product name: Recombinant Mouse TIM4 protein (rFc Tag) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 23-279 aa of NM_178759 Sequence: ASEDTIIGFLGQPVTLPCHYLSWSQSRNSMCWGKGSCPNSKCNAELLRTDGTRIISRKSTKYTLLGKVQFGEVSLTISNTNRGDSGVYCCRIEVPGWFNDVKKNVRLELRRATTTKKPTTTTRPTTTPYVTTTTPELLPTTVMTTSVLPTTTPPQTLATTAFSTAVTTCPSTTPGSFSQETTKGSAFTTESETLPASNHSQRSMMTISTDIAVLRPTGSNPGILPSTSQLTTQKTTLTTSESLQKTTKSHQINSRQT Predict reactive species
Full Name	T-cell immunoglobulin and mucin domain containing 4
Calculated Molecular Weight	38 kDa
GenBank Accession Number	NM_178759
Gene Symbol	TIM4
Gene ID (NCBI)	276891
RRID	AB_3746044
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q6U7R4
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

T cell immunoglobulin mucin 4 (TIM4) is a macrophage receptor for phosphatidylserine (PtdSer) on apoptotic cells. It is evidenced that TIM4 as phosphatidylserine (PS) receptor is capable of maintaining peritoneal macrophages' homeostatic function and regulating adaptive immunity based on the alteration of Ag-particular T cells' clearance. It is noteworthy that TIM4's dysregulating process was identified under several autoimmune states, in particular inside immune cells. In addition, TIM4 was identified to be over-expressed in Systemic lupus erythematosus (SLE) cases.