Tested Applications
| Positive FC detected in | Transfected HEK-293T cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
98424-3-RR targets uPAR/CD87 in IF, FC applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg3156 Product name: Recombinant Mouse uPAR/CD87 protein (rFc Tag) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 24-297 aa of NM_011113.4 Sequence: LQCMQCESNQSCLVEECALGQDLCRTTVLREWQDDRELEVVTRGCAHSEKTNRTMSYRMGSMIISLTETVCATNLCNRPRPGARGRAFPQGRYLECASCTSLDQSCERGREQSLQCRYPTEHCIEVVTLQSTERSLKDEDYTRGCGSLPGCPGTAGFHSNQTFHFLKCCNYTHCNGGPVLDLQSFPPNGFQCYSCEGNNTLGCSSEEASLINCRGPMNQCLVATGLDVLGNRSYTVRGCATASWCQGSHVADSFPTHLNVSVSCCHGSGCNSPT Predict reactive species |
| Full Name | plasminogen activator, urokinase receptor |
| Calculated Molecular Weight | 35 kDa |
| GenBank Accession Number | NM_011113.4 |
| Gene Symbol | Plaur |
| Gene ID (NCBI) | 18793 |
| RRID | AB_3746064 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P35456 |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2 - 8°C. Stable for one year after shipment. |
Background Information
uPAR, also known as CD87, is a highly glycosylated, GPI-anchored membrane protein. In addition to the membrane-anchored form, uPAR is released from the plasma membrane by cleavage of the GPI anchor and can be found as a soluble form (suPAR). uPAR contains three homologous domains (D1-D3) of which the N-terminal one (D1) represents the uPA-binding domain. After binding to uPAR, uPA cleaves plasminogen, generating the active protease plasmin which is involved in a wide variety of physiologic and pathologic processes. In addition to regulating proteolysis, uPAR has important function in cell adhesion, migration and proliferation. Studies reveal that uPAR expression is elevated during inflammation and tissue remodeling and in many human cancers, in which it frequently indicates poor prognosis. (PMID 20027185; 12461559)
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for uPAR/CD87 antibody 98424-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Anti-Mouse uPAR/CD87 Rabbit Recombinant Antibody (242976A9), catalog number 98424-3-RR, has a total of 1 citation. The cited publication appeared in Advanced Healthcare Materials (IF: 11), published in 2026. The research focuses on cartilage senescence and cellular uptake, specifically investigating how clustering of urokinase-type plasminogen activator receptor (uPAR) in hydrogel microspheres can remodel cellular uptake to alleviate cartilage aging. The antibody was applied in flow cytometry and immunofluorescence for mouse samples.
| Species | Application | Title |
|---|---|---|
Adv Healthc Mater Remodeling Cellular Uptake via Inducing Clustering of Urokinase Receptors in Hydrogel Microspheres for Alleviating Cartilage Senescence. |
