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Anti-Human Neutrophil Elastase Rabbit Recombinant Antibody (98499-1-RR)

Anti-Human Neutrophil Elastase Rabbit Recombinant Antibody

Neutrophil Elastase Uni-rAb^® Recombinant Antibody for FC (Intra)

Cat No. 98499-1-RR

Clone No. 250346G5

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

ELA2, ELANE, EC:3.4.21.37, Elastase 2, HLE

Formulation: PBS, Azide

PBS, Azide

PBS Only

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 647 CoraLite®594

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Filter: AllFC (Intra)

FC experiment of human peripheral blood leukocytes using 98499-1-RR
1x10^6 human peripheral blood leukocytes were intracellularly stained with 0.25 ug Anti-Human Neutrophil Elastase Rabbit RecAb (98499-1-RR, Clone: 250346G5) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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1x10^6 human peripheral blood leukocytes were intracellularly stained with 0.25 ug Anti-Human Neutrophil Elastase Rabbit RecAb (98499-1-RR, Clone: 250346G5) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.
FC experiment of U-937 using 98499-1-RR
1x10^6 U-937 cells (left) or Jurkat cells (right) were intracellularly stained with 0.25 ug Anti-Human Neutrophil Elastase Rabbit RecAb (98499-1-RR, Clone: 250346G5) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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What's the best dilution for Neutrophil Elastase in ?

Overview

Tested Applications
Product Information
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MSDS SDS

Tested Applications

Positive FC (Intra) detected in

human peripheral blood leukocytes, U-937 cells

Recommended dilution

Application	Dilution
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98499-1-RR targets Neutrophil Elastase in FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg3192 Product name: Recombinant Human Neutrophil Elastase protein (rFc Tag) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 28-267 aa of NM_001972.4 Sequence: SEIVGGRRARPHAWPFMVSLQLRGGHFCGATLIAPNFVMSAAHCVANVNVRAVRVVLGAHNLSRREPTRQVFAVQRIFENGYDPVNLLNDIVILQLNGSATINANVQVAQLPAQGRRLGNGVQCLAMGWGLLGRNRGIASVLQELNVTVVTSLCRRSNVCTLVRGRQAGVCFGDSGSPLVCNGLIHGIASFVRGGCASGLYPDAFAPVAQFVNWIDSIIQRSEDNPCPHPRDPDPASRTH Predict reactive species
Full Name	elastase 2, neutrophil
Calculated Molecular Weight	29KD
GenBank Accession Number	NM_001972.4
Gene Symbol	ELA2
Gene ID (NCBI)	1991
RRID	AB_3746129
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P08246
Storage Buffer	PBS with 0.09% sodium azide, pH 7.3.
Storage Conditions	Store at 2 - 8°C. Stable for one year after shipment.

Background Information

ELA2(Neutrophil elastase) is also named as ELAL, NE, HLE, HNE, PMN-E, SERP1, GE, ELANE and belongs to the peptidase S1 family. It is a 33-kDa enzyme with several isoforms that differ in their extent of glycosylation (PMID:12223222). Human neutrophil elastase (HNE) is a serine protease with potent proteolytic activity, which is reported to cause mucin secretion (degranulation) (PMID:12169572). It may contribute to the directed migration of leukocytes into flamed postcapillary venules in addition to contributing to the process of tissue emigration (PMID:9683424). The full length protein has a signal peptide,propeptide and three glycosylation sites.