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Anti-Mouse IL-28B Rabbit Recombinant Antibody (98567-3-RR)

Anti-Mouse IL-28B Rabbit Recombinant Antibody

IL-28B Uni-rAb^® Recombinant Antibody for FC (Intra)

Cat No. 98567-3-RR

Clone No. 250563C9

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Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra)

Synonyms

Ifnl3, IFN-lambda-3, Il28, Il28b, Interferon lambda-3

Formulation: PBS, Azide

PBS, Azide

PBS Only

Conjugate: Unconjugated

Unconjugated

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Filter: AllFC (Intra)Affinity

FC experiment of Transfected HEK-293T using 98567-3-RR
1x10^6 HEK-293T cells (left) or IL-28B transfected HEK-293T cells (right) were stained with 0.25 ug Anti-Mouse IL-28B Rabbit RecAb (98567-3-RR, Clone: 250563C9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of Transfected HEK-293T using 98567-3-RR
1x10^6 HEK-293T cells (left) or IL-28B transfected HEK-293T cells (right) were stained with 0.25 ug Anti-Mouse IL-28B Rabbit RecAb (98567-3-RR, Clone: 250563C9) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9)(blue), and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Affinity and Kinetic Characterization of 98567-3-RR
Biolayer interferometry (BLl) kinetic assays of 98567-3-RR against Mouse IL-28B were performed. The affinity constant is below 1 pM.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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What's the best dilution for IL-28B in ?

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Tested Applications
Product Information
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MSDS SDS

Tested Applications

Positive FC (Intra) detected in

Transfected HEK-293T cells

Recommended dilution

Application	Dilution
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98567-3-RR targets IL-28B in FC (Intra) applications and shows reactivity with mouse samples.

Tested Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg3146 Product name: Recombinant Mouse IL-28B/ IFNL3 protein (rFc Tag) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 20-193 aa of NM_177396.1 Sequence: DPVPRATRLPVEAKDCHIAQFKSLSPKELQAFKKAKGAIEKRLLEKDMRCSSHLISRAWDLKQLQVQERPKALQAEVALTLKVWENINDSALTTILGQPLHTLSHIHSQLQTCTQLQATAEPKPPSRRLSRWLHRLQEAQSKETPGCLEDSVTSNLFQLLLRDLKCVASGDQCV Predict reactive species
Full Name	interleukin 28B
Calculated Molecular Weight	22 kDa
GenBank Accession Number	NM_177396.1
Gene Symbol	Il28b
Gene ID (NCBI)	338374
RRID	AB_3746184
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q8CGK6
Storage Buffer	PBS with 0.09% sodium azide, pH 7.3.
Storage Conditions	Store at 2 - 8°C. Stable for one year after shipment.

Background Information

IL-28B is a type III IFN, which shares many of the biological effects of type I IFNs but may have fewer side effects due to a more selective receptor distribution. It interacts with a heterodimeric class II cytokine receptor that consists of interleukin 10 receptor, beta (IL10RB) and interleukin 28 receptor, alpha (IL28RA) (PMID: 20712453;12469119;16539846).