Home
Products
Primary Antibodies
Flow Cytometry Antibodies
Anti-Human IgA1 Rabbit Recombinant Antibody (98758-3-RR)

Anti-Human IgA1 Rabbit Recombinant Antibody

IgA1 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98758-3-RR

Clone No.252592G2

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Synonyms

Human IgA, Human IgA Heavy Chain, IGHA1, IgA, Igh2

Formulation: PBS, Azide

PBS, Azide

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Filter: AllFC

FC experiment of human PBMCs using 98758-3-RR
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human IgA1 Rabbit RecAb (98758-3-RR, Clone: 252592G2) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 488 Anti-Mouse CD4 (GK1.5) Rat Recombinant Antibody (CL488-65662, Clone: GK1.5). The cells originate from CD11b-negative cells. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human IgA1 Rabbit RecAb (98758-3-RR, Clone: 252592G2) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 488 Anti-Mouse CD4 (GK1.5) Rat Recombinant Antibody (CL488-65662, Clone: GK1.5). The cells originate from CD11b-negative cells. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

Overview

Tested Applications
Product Information
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive FC detected in

human PBMCs

Recommended dilution

Application	Dilution
Flow Cytometry (FC)	FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98758-3-RR targets IgA1 in FC applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg9190 Product name: Recombinant human IgA1-IgK protein Source: mammalian cells-derived, pHZ-KIsec-C-6HIS Tag: C-6HIS Domain: 10011193 of Sequence: \ Predict reactive species
Full Name	immunoglobulin heavy constant alpha 1
Gene Symbol	Human IgA Heavy Chain
Gene ID (NCBI)	3493
RRID	AB_3746325
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
Storage Buffer	PBS with 0.09% sodium azide, pH 7.3.
Storage Conditions	Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Immunoglobulins (Ig), also known as antibodies, are glycoproteins produced by B cells and plasma cells. There are 5 main classes of mammalian immunoglobulin heavy chains. Each class defines the IgM, IgG, IgA, IgD, and IgE isotypes (PMID: 20176268). IgA is the most prevalent antibody class at mucosal sites with an important role in mucosal defense. There are 2 subclasses of IgA, IgA1 and IgA2, which differ over a stretch of 18 amino acids in their hinge region, with the hinge of IgA1 being 13 amino acids longer than that of IgA2 (PMID: 21691145). This antibody specifically recognizes human IgA1 and does not cross-react with IgA2.