Anti-Human IgA1 Rabbit Recombinant Antibody, PBS Only

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• Anti-Human IgA1 Rabbit Recombinant Antibody, PBS Only (98758-3-PBS)

IgA1 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98758-3-PBS

Clone No.252592G2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

Human IgA, Human IgA Heavy Chain, IGHA1, IgA, Igh2

• 

  FC experiment of human PBMCs using 98758-3-RR (same clone as 98758-3-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human IgA1 Rabbit RecAb (98758-3-RR, Clone: 252592G2) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 488 Anti-Mouse CD4 (GK1.5) Rat Recombinant Antibody (CL488-65662, Clone: GK1.5). The cells originate from CD11b-negative cells. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98758-3-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

98758-3-PBS targets IgA1 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg9190

  Product name: Recombinant human IgA1-IgK protein

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 10011193 of

  Sequence: \

• Full Name: immunoglobulin heavy constant alpha 1
• Gene Symbol: Human IgA Heavy Chain
• Gene ID (NCBI): 3493
• RRID: AB_3746325
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Immunoglobulins (Ig), also known as antibodies, are glycoproteins produced by B cells and plasma cells. There are 5 main classes of mammalian immunoglobulin heavy chains. Each class defines the IgM, IgG, IgA, IgD, and IgE isotypes (PMID: 20176268). IgA is the most prevalent antibody class at mucosal sites with an important role in mucosal defense. There are 2 subclasses of IgA, IgA1 and IgA2, which differ over a stretch of 18 amino acids in their hinge region, with the hinge of IgA1 being 13 amino acids longer than that of IgA2 (PMID: 21691145). This antibody specifically recognizes human IgA1 and does not cross-react with IgA2.