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Anti-Human C-peptide Rabbit Recombinant Antibody (98867-1-RR)

Anti-Human C-peptide Rabbit Recombinant Antibody

C-peptide Uni-rAb^® Recombinant Antibody for IHC, FC (Intra)

Cat No. 98867-1-RR

Clone No.253514D7

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, FC (Intra)

Synonyms

C peptide, INS C-Peptide, Insulin C-Peptide

Formulation: PBS, Azide

PBS, Azide

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

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Freight/Packing: -

Quantity

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Filter: AllIHCFC (Intra)

IHC staining of human pancreas using 98867-1-RR
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 98867-1-RR (C-Peptide antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human pancreas using 98867-1-RR
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 98867-1-RR (C-Peptide antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of Transfected CHO using 98867-1-RR
1x10^6 CHO cells or C-Peptide transfected CHO cells were intracellularly stained with 0.25 ug Anti-Human C-Peptide Rabbit RecAb (98867-1-RR, Clone: 253514D7) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive IHC detected in	human pancreas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive FC (Intra) detected in	Transfected CHO cells

Recommended dilution

Application	Dilution
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98867-1-RR targets C-peptide in IHC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	INS
Calculated Molecular Weight	110aa, 12 kDa
GenBank Accession Number	NM_000207
Gene Symbol	INS
Gene ID (NCBI)	3630
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P01308
Storage Buffer	PBS with 0.09% sodium azide, pH 7.3.
Storage Conditions	Store at 2 - 8°C. Stable for one year after shipment.

Background Information

C-peptide (connecting peptide) is a short 31-amino-acid polypeptide that connects insulin's A-chain to its B-chain in the proinsulin molecule. It is cleaved from proinsulin in the pancreatic beta cells, and one molecule of C-peptide is released for every molecule of insulin (PMID: 6759081). C-peptide is secreted at a relatively steady rate over long duration, making it an effective biomarker for endogenous insulin secretion (PMID: 41694179).