Anti-Human C-peptide Rabbit Recombinant Antibody, PBS Only

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• Anti-Human C-peptide Rabbit Recombinant Antibody, PBS Only (98867-1-PBS)

C-peptide Uni-rAb^® Recombinant Antibody for IHC, FC (Intra)

Cat No. 98867-1-PBS

Clone No.253514D7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, FC (Intra)

Synonyms

C peptide, INS C-Peptide, Insulin C-Peptide

• 

  IHC staining of human pancreas using 98867-1-RR (same clone as 98867-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 98867-1-RR (C-Peptide antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 98867-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas using 98867-1-RR (same clone as 98867-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 98867-1-RR (C-Peptide antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 98867-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of Transfected CHO using 98867-1-RR (same clone as 98867-1-PBS)

  1x10^6 CHO cells or C-Peptide transfected CHO cells were intracellularly stained with 0.25 ug Anti-Human C-Peptide Rabbit RecAb (98867-1-RR, Clone: 253514D7) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). This data was developed using the same antibody clone with 98867-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

98867-1-PBS targets C-peptide in IHC, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: INS
• Calculated Molecular Weight: 110aa, 12 kDa
• GenBank Accession Number: NM_000207
• Gene Symbol: INS
• Gene ID (NCBI): 3630
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P01308
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

C-peptide (connecting peptide) is a short 31-amino-acid polypeptide that connects insulin's A-chain to its B-chain in the proinsulin molecule. It is cleaved from proinsulin in the pancreatic beta cells, and one molecule of C-peptide is released for every molecule of insulin (PMID: 6759081). C-peptide is secreted at a relatively steady rate over long duration, making it an effective biomarker for endogenous insulin secretion (PMID: 41694179).