Tested Applications
| Positive FC detected in | human PBMCs, Raji cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 5 ul per 10^6 cells in 100 μl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
APC-65110 targets CD19 in FC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
N/A Predict reactive species |
| Full Name | CD19 molecule |
| Calculated Molecular Weight | 556 aa, 61 kDa |
| GenBank Accession Number | BC006338 |
| Gene Symbol | CD19 |
| Gene ID (NCBI) | 930 |
| ENSEMBL Gene ID | ENSG00000177455 |
| RRID | AB_2882999 |
| Conjugate | APC Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 650 nm / 660 nm |
| Excitation Laser | Red Laser (633 nm) |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | P15391 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for APC CD19 antibody APC-65110 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
APC-65110 is the Anti-Human CD19 (HIB19) antibody, with 2 total citations. One citation appears in Advanced Science (2025), studying tendon regeneration and the secretome of adipose-derived stem cells. The other is in Clinical, Cosmetic and Investigational Dermatology (2026), examining elevated IgE levels and Th2 dominance in psoriasis with overlapping atopic dermatitis features. The associated research fields are stem cell biology/tissue regeneration and immunodermatology/allergic inflammation.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Enhancing Tendon Regeneration: Investigating the Impact of Topography on the Secretome of Adipose-Derived Stem Cells | ||
Clin Cosmet Investig Dermatol Elevated IgE Levels in Psoriasis: An Atypical Subtype with Th2 Dominance and Overlapping Features of Atopic Dermatitis. |
