Recombinant Human IL-1 Beta protein (His Tag)
Species
Human
Purity
>90 %, SDS-PAGE
Tag
His Tag
Activity
not tested
Cat no : Eg0286
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human IL-1 Beta protein Ala117-Ser269 (Accession# P01584 ) with a His tag at the N-terminus. |
| GeneID | 3553 |
| Accession | P01584 |
| PredictedSize | 18.2 kDa |
| SDS-PAGE | 19-20 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1 beta and their naturally occurring inhibitor IL-1RN. Interleukin 1 beta (IL-1 beta), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1 beta is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1 beta is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions.
References:
1. Xu, Jiali et al. PloS one vol. 8,5 e63654. 21 May. 2013. 2. McCarty, Stephanie, and William Frishman. Cardiology in review vol. 22,4 (2014): 176-81. 3. Dinarello, C A. Blood vol. 87,6 (1996): 2095-147. 4. Bird, Steve et al. Cytokine & growth factor reviews vol. 13,6 (2002): 483-502.
