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Recombinant Mouse CD45 protein (His Tag)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

His Tag

Activity

not tested

Cat no : Eg0845

Validation Data Gallery

  • Purity of Recombinant Mouse CD45 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse CD45 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression CHO-derived Mouse CD45 protein Gln26-Lys566 (Accession# P06800-4) with a His tag at the C-terminus.
GeneID 19264
Accession P06800-4
PredictedSize 59.8 kDa
SDS-PAGE 85-140 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets. CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling.

References:

1. F W Shen, et al. (1986) Immunogenetics. 24(3):146-9.
2. J G Altin, et al. (1997) Review Immunol Cell Biol. 75(5):430-45.
3. Nicholas D Huntington, et al. (2006) Nat Immunol. 7(2):190-8. 
4. Salema Jafri, et al. (2017) Sci Rep. 7(1):3495.