Recombinant Human GDF8/Myostatin protein (rFc Tag)
Species
Human
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg1972
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human GDF8 protein Asn24-Ser375 (Accession# O14793) with a rabbit IgG Fc tag at the N-terminus. |
| GeneID | 2660 |
| Accession | O14793 |
| PredictedSize | 66.9 kDa |
| SDS-PAGE | 13 kDa, 58-65kDa and 65-85 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
Myostatin, also known as GDF-8, is a member of the TGF-beta superfamily. Myostatin is expressed in the myotome and developing skeletal muscles. Myostatin negatively regulates skeletal muscle growth and development through the regulation of anabolic and catabolic pathways in skeletal muscles. Myostatin immunoreactive bands could be identified at ∼50, 32-35, and 16 kDa, which correspond to the precursor, glycosylated dimmer, and monomer, respectively. GDF8 may have cross-reactions with GDF11.
References:
1. Jespersen, J G et al. Scandinavian journal of medicine & science in sports vol. 21,2 (2011) 2. Wehling, M et al. FASEB journal : official publication of the Federation of American Societies for Experimental Biology vol. 14,1 (2000) 3. George, Isaac et al. European journal of heart failure vol. 12,5 (2010): 444-53.