Recombinant Mouse IL-3RB/CD131 protein (rFc Tag)
Species
Mouse
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg2173
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Mouse IL-3RB protein His23-Trp441 (Accession# P26955) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 12983 |
| Accession | P26955 |
| PredictedSize | 74.7 kDa |
| SDS-PAGE | 70-90 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
CD131, also known as IL-3RB, IL-5RB, colony stimulating factor 2 receptor subunit beta (CSF2RB), or cytokine receptor common subunit beta, is the common subunit of the type I cytokine receptors for granulocyte-macrophage colony-stimulating factor (GM-CSF), IL-3 and IL-5. It is expressed predominantly by hematopoietic cells but also by non-hematopoietic cells. CD131 mediates intracellular signaling by JAK2, leading to phosphorylation of CD131 and triggering signaling pathways such as STAT5, MAPK and PI3-Kinase/AKT which regulate cell survival, proliferation and function.
References:
1. Beatriz F Côrte-Real, et al. (2022) Front Immunol. 13:1005965. 2. C L Scott, et al. (1999) Int J Biochem Cell Biol. 31(10):1011-5. 3. Timothy R Hercus, et al. (2013) Cytokine Growth Factor Rev. 24(3):189-201.