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Recombinant Mouse SEMA5A protein (rFc Tag) (HPLC verified)

Species

Mouse

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg2689

Validation Data Gallery

  • Purity of Recombinant Mouse SEMA5A was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse SEMA5A was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • The purity of Mouse SEMA5A was greater than 90% as determined by SEC-HPLC.

    The purity of Mouse SEMA5A was greater than 90% as determined by SEC-HPLC.

Product Information

Purity >90 %, SDS-PAGE
>90 %, SEC-HPLC
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Mouse SEMA5A protein Glu23-Thr765 (Accession# Q3UPZ0) with a rabbit IgG Fc tag at the C-terminus.
GeneID 20356
Accession Q3UPZ0
PredictedSize 109.6 kDa
SDS-PAGE 110-130 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Semaphorin 5A, is a member of the semaphorin family of proteins, which are known for their roles in axon guidance and the development of the nervous system.Sema5A is a bifunctional guidance cue that can exert both permissive and inhibitory properties toward developing axons and migrating cells. The protein is also implicated in the promotion of angiogenesis by increasing endothelial cell proliferation, migration, and inhibiting apoptosis.

References:

1. Nagy GN. et al. (2024). Nat Commun.15(1):2723. 
2. Sadanandam A. et al. (2010). Microvasc Res. 79(1):1-9.
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