Recombinant Human FCGR2A/CD32a protein (rFc Tag)
Species
Human
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg2713
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human FCGR2A protein Gln34-Gly217 (Accession# P12318-1) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 2212 |
| Accession | P12318-1 |
| PredictedSize | 46.4 kDa |
| SDS-PAGE | 50-60 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
IgG Fc receptors (FcγRs) play important roles in immune responses. Low-affinity IgG Fc receptor Fc gamma RIIa (FCGR2A, CD32a) is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils and is involved in the process of phagocytosis and clearing of immune complexes.
References:
1.Gessner JE, et al. (1998) Ann Hematol.76(6):231-48. 2.Anania JC, et al. (2019) Front Immunol.19;10:464.