Recombinant Human CD320 protein (rFc Tag)
Species
Human
Purity
>90 %, SDS-PAGE
Tag
C-rFc
Activity
not tested
Cat no : Eg2795
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human CD320 protein Ser36-Tyr229 (Accession# Q9NPF0-1) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 51293 |
| Accession | Q9NPF0-1 |
| PredictedSize | 46.0 kDa |
| SDS-PAGE | 55-70 kDa, reducing (R) condition |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
CD320, also known as 8D6, 8D6A, or TCblR, is a single-pass type I membrane protein containing 2 LDL-receptor class A domains. Expressed abundantly on follicular dendritic cells (FDCs), CD320 has been shown to enhance the proliferation of germinal center (GC) B cells. It is the receptor for cellular uptake of transcobalamin-bound cobalamin. Defects in CD320 are the cause of methylmalonic aciduria type TCblR (MMATC).
References:
1. Edward V Quadros. et al. (2009). Blood. 113(1):186-192. 2. Edward V Quadros. et al.(2013). Biochimie. 95(5):1008-1018. 3. Quadros EV. et al. (2005). Biochem Biophys Res Commun. 327:1006-1010.