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Recombinant Human IFNAR2 protein (rFc Tag) (HPLC verified)

Species

Human

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg2928

Validation Data Gallery

  • Purity of Recombinant Human IFNAR2 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human IFNAR2 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • The purity of Human IFNAR2 was greater than 90% as determined by SEC-HPLC.

    The purity of Human IFNAR2 was greater than 90% as determined by SEC-HPLC.

Product Information

Purity >90 %, SDS-PAGE
>90 %, SEC-HPLC
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human IFNAR2 protein Ile27-Lys243 (Accession# P48551-1) with a rabbit IgG Fc tag at the C-terminus.
GeneID 3455
Accession P48551-1
PredictedSize 50.8 kDa
SDS-PAGE 55-80 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

IFNAR2 is a type I membrane protein and is part of one of the two chains of a receptor for interferon (IFN), alpha and beta. IFNAR2 has three isoforms, the first of which is a non-functional protein with a truncated cytoplasmic domain. The second is lengthy and includes the functional trans-membrane protein together with IFNAR1. The soluble version of the receptor is the third form (sIFNAR2).

References:

1. Órpez-Zafra T. et al. (2017). Mult. Scler. J. 23:937–945.
2. López-Bielma MF. et al. (2023) Pathogens. 12(11):1320.