Recombinant Mouse S100A8 protein (rFc Tag)
Species
Mouse
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg2959
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Mouse S100A8 protein Pro2-Glu89 (Accession# P27005) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 20201 |
| Accession | P27005 |
| PredictedSize | 35.7 kDa |
| SDS-PAGE | 37-47 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
S100A8 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. S100A8 may form homodimer or heterodimer with S100A9(16216873).
References:
1. Li Y, Chen B, et al. (2019) Circulation. 140(9):751-764. 2. Tousif S, Singh AP, et al. (2023) Circ Res. 132(3):267-289. 3. Cho E, Mun SJ, et al. (2024) Acta Pharmacol Sin. 45(3):581-593. 4. Guo Q, Zhao Y, et al. (2021) Cell Host Microbe. 29(2):222-235.e4.