Recombinant Human PDIA4/ERp72 protein (rFc Tag)
Species
Human
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg3011
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human PDIA4 protein Val21-Thr641 (Accession# P13667) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 9601 |
| Accession | P13667 |
| PredictedSize | 96.2 kDa |
| SDS-PAGE | 85-110 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
PDIA4(Protein disulfide-isomerase A4) is also named as ERP70, ERP72 and belongs to the protein disulfide isomerase family. It catalyzes the rearrangement of -S-S- bonds in proteins. ERp72 is a soluble protein localized in the ER lumen and contains the COOH-terminal retention signal, KEEL. There are 6 cysteine residues in the amino acid sequences of mouse and human ERp72. All of the cysteine residues occur in the internal thioredoxin motif, CGHC(PMID:15475357). The full length protein has a signal peptide with 20 amino acid.
References:
1. van Lith M. et al. (2005). J Biol Chem. 280(2):1376-83.