Recombinant Human Pancreatic alpha-amylase protein (rFc Tag)
Species
Human
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg3071
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human Pancreatic alpha-amylase protein Gln16-Leu511 (Accession# P04746-1) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 279 |
| Accession | P04746-1 |
| PredictedSize | 81.9 kDa |
| SDS-PAGE | 75-90 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
AMY2A, or amylase alpha 2A, is a gene in humans that encodes for a specific isoenzyme of the alpha-amylase family of proteins. AMY2A is expressed at high levels in the pancreas and is selectively found in the cytoplasm of pancreatic exocrine glandular cells. This protein is a secreted enzyme that plays a crucial role in the digestive process, particularly in the breakdown of dietary starch and glycogen.
References:
1. Nakajima K. (2016). World J Diabetes. 25;7(6):112-21. 2. Hariharan R. et al. (2021). Obes Rev. 22(6):e13205.