Recombinant Human Angiotensinogen/AGT protein (rFc Tag)
Species
Human
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg3099
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human Angiotensinogen protein Asp25-Ala476 (Accession# P01019) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 183 |
| Accession | P01019 |
| PredictedSize | 75.8 kDa |
| SDS-PAGE | 75-85 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
Angiotensinogen(AGT), a precursor of angiotensin II (Ang II), is expressed and synthesized largely in the liver and cleaved by the enzyme renin in response to lowered blood pressure. It has a key role in mediating vascular constriction and regulating salt and fluid homeostasis. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development.
References:
1. Hong Lu. et al. (2016). Hypertens Res.39(7):492-500. 2. Tanvir Kahlon. et al.(2022). JACC Heart Fail.10(10):699-713. 3. M Caulfield. et al. (1996). Hypertension. 28(6):1123-5. 4. L Morgan. et al. (1996). Int J Biochem Cell Biol. 28(11):1211-22.