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Recombinant Mouse IL-4 protein (rFc Tag)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg3750

Validation Data Gallery

  • Purity of Recombinant Mouse IL-4 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse IL-4 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Mouse IL-4 protein His21-Ser140 (Accession# P07750) with a rabbit IgG Fc tag at the C-terminus.
GeneID 16189
Accession P07750
PredictedSize 39.8 kDa
SDS-PAGE 40-55 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Interleukin-4 (IL-4), a member of the α-helical cytokine family, is produced by activated CD4 + T cells, basophils, and mast cells. It promotes the proliferation and differentiation of antigen presenting cells. IL-4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis. IL-4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival.

References:

1. Scheller J. et al. (1988). Behring Inst Mitt. Behring Inst Mitt. (83):40-7.
2. Heinrich PC. et al. (2003). Biochem J. 374: 1-20.
3. Rose-John S. et al. (2007). Expert Opin Ther Targets. 11: 613-24.
4. Ming JE. et al. (1989). J Mol Cell Immunol. 4: 203-11.
5. Febbraio MA. et al. (2005). Exerc Sport Sci Rev. 33:114-9.
6. J Appl Physiol. et al. (2005). J Appl Physiol. 98:1154-62.