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Recombinant Rat CD80 protein (rFc Tag) (HPLC verified)

Species

Rat

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg3841

Validation Data Gallery

  • Purity of Recombinant Rat CD80 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Rat CD80 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • The purity of Rat CD80 was greater than 90% as determined by SEC-HPLC.

    The purity of Rat CD80 was greater than 90% as determined by SEC-HPLC.

Product Information

Purity >90 %, SDS-PAGE
>90 %, SEC-HPLC
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Rat CD80 protein Ile39-Gln248 (Accession# Q62680) with a rabbit IgG Fc tag at the C-terminus.
GeneID 25408
Accession Q62680
PredictedSize 50.2 kDa
SDS-PAGE 60-75 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD80, also known as B7, B7.1, or BB1, is a member of the immunoglobulin superfamily with a size of 44-54 kDa and is expressed by a variety of cells, including activated B cells, T cells, macrophages, dendritic cells, and tumor cells. CD80 plays a crucial role in T cell activation.

References:

1. Hathcock KS, et al. J Exp Med. (1994) 180:631–40.
2. Freeman GJ, et al. J Immunol. (1989) 143:2714–22.
3. Li L, et al. Front Immunol. 2024 Nov 7;15:1496992.
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