Recombinant Human LAMP2/ CD107b protein (rFc Tag)
Species
Human
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg4998
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human LAMP2 protein Leu29-Phe375 (Accession# P13473-1) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 3920 |
| Accession | P13473-1 |
| PredictedSize | 64.6 kDa |
| SDS-PAGE | 80-110 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis. Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells. LAMP2 is involved in lysosomal stability and autophagy. This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may play a role in tumor cell metastasis.
References:
1. R Kundra, S Kornfeld. (1999) J Biol Chem. Oct 22;274(43):31039-46. 2. Eeva-Liisa Eskelinen, et al. (2002) Mol Biol Cell. Sep;13(9):3355-68. 3. V Sarafian, et al. (1998) Int J Cancer. Jan 5;75(1):105-11.