Recombinant Human CIB1 protein (rFc Tag)
Species
Human
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg5476
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human CIB1 protein Gly2-Leu191 (Accession# Q99828-1) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 10519 |
| Accession | Q99828-1 |
| PredictedSize | 47.2 kDa |
| SDS-PAGE | 43-50 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
CIB1(Calcium and integrin-binding protein 1) is also named as CIB, KIP, PRKDCIP. It is an EF-hand-containing protein that binds multiple effector proteins, including the platelet αIIbβ3 integrin and several serine/threonine kinases and potentially modulates their function. It regulates platelet aggregation in hemostasis through a specific interaction with the alphaIIb cytoplasmic domain of platelet integrin alphaIIbbeta3.
References:
1. Leisner, Tina M., et al. The FASEB Journal 30.8 (2016): 2640. 2. Wang, Xianwang, et al. Cellular Physiology and Biochemistry 43.4 (2017): 1413-1424. 3. Heineke, Joerg, et al. Nature medicine 16.8 (2010): 872-879. 4. Yoon, Kyoung Wan, et al. Proceedings of the National Academy of Sciences 106.41 (2009): 17389-17394. 5. Zayed, Mohamed A., et al. Circulation research 101.11 (2007): 1185-1193.