Recombinant Mouse CD33 protein (rFc Tag)
Species
Mouse
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg6285
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Mouse CD33 protein Asp18-Glu240 (Accession# Q63994-1) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 12489 |
| Accession | Q63994-1 |
| PredictedSize | 50.2 kDa |
| SDS-PAGE | 58-68 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
CD33, also referred to as Siglec-3, is a 67-kDa glycosylated transmembrane protein of sialic acid-binding immunoglobulin-like lactic (siglec) family. CD33 is expressed on monocytes, myeloid progenitors, granulocytes, mast cells, some T cells. It may mediate cell-to-cell adhesion and act as a receptor that inhibits the proliferation of normal and leukemic myeloid cells.
References:
1. Brinkman-Van der Linden, Els C M et al. Molecular and cellular biology vol. 23,12 (2003): 4199-206. 2. Tan, Shuwen et al. Neuromolecular medicine vol. 25,2 (2023): 255-271. 3. Griciuc, Ana et al. Human molecular genetics vol. 29,17 (2020): 2920-2935.