Recombinant Rat Clusterin protein (rFc Tag)
Species
Rat
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg9471
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Rat Clusterin protein Glu22-Glu447 (Accession# P05371) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 24854 |
| Accession | P05371 |
| PredictedSize | 74.7 kDa |
| SDS-PAGE | 27-33 kDa, 58-70 kDa and 85-95 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
Clusterin (also known as apolipoprotein J) is a 75-80 kDa heterodimeric glycoprotein produced by a wide array of tissues and found in most biological fluids. It is encoded by one single gene and cleaved posttranslationally into α and β subunits prior to secretion from the cell. Many functions have been described for clusterin, including regulating apoptosis, transporting lipids, controlling cell interactions, and regulating complement. Increased plasma clusterin levels have been associated with the severity, pathology, and progression of Alzheimer's disease.
References:
1. Rosenberg, M E, and J Silkensen. The international journal of biochemistry & cell biology vol. 27,7 (1995): 633-45. 2. Wilson, M R, and S B Easterbrook-Smith. Trends in biochemical sciences vol. 25,3 (2000): 95-8. 3. Jones, Steve E, and Catherine Jomary. The international journal of biochemistry & cell biology vol. 34,5 (2002): 427-31. 4. Thambisetty, Madhav et al. Archives of general psychiatry vol. 67,7 (2010): 739-48. 5. Schrijvers, Elisabeth M C et al. JAMA vol. 305,13 (2011): 1322-6.