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Mouse CCL17/TARC Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: mouse

Detection: mouse

Applications

Sandwich ELISA

Range

Sandwich ELISA: 23.4-1500 pg/mL

Cat no : MP02817-1

Synonyms

CCL17, TARC, ABCD-2, C C motif chemokine 17, CC chemokine TARC

Validation Data Gallery

  • Sandwich ELISA standard curve of MP02817-1, Mouse CCL17/TARC Recombinant Matched Antibody Pair - PBS only. 86482-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg3319. 86482-2-PBS was HRP conjugated as the detection antibody. Range: 23.4-1500 pg/mL.

    Sandwich ELISA standard curve of MP02817-1

    Sandwich ELISA standard curve of MP02817-1, Mouse CCL17/TARC Recombinant Matched Antibody Pair - PBS only. 86482-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg3319. 86482-2-PBS was HRP conjugated as the detection antibody. Range: 23.4-1500 pg/mL.

  • Serum of eleven mice was measured. The CCL17/TARC concentration of detected samples was determined to be 244.4 pg/mL with a range of 16.2-496.4 pg/mL.

    Sandwich ELISA sample test of MP02817-1

    Serum of eleven mice was measured. The CCL17/TARC concentration of detected samples was determined to be 244.4 pg/mL with a range of 16.2-496.4 pg/mL.

  • Mouse lung, cut into 1-2 mm pieces, was cultured in RPMI 1640 supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernatant was removed, assayed for CCL17/TARC, and measured 952.8 pg/mL.

    Sandwich ELISA sample test of MP02817-1

    Mouse lung, cut into 1-2 mm pieces, was cultured in RPMI 1640 supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernatant was removed, assayed for CCL17/TARC, and measured 952.8 pg/mL.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP02817-1 targets CCL17/TARC in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 23.4-1500 pg/mL (Sandwich ELISA);

Capture antibody: Mouse Ccl17 Recombinant monoclonal antibody, PBS Only (Capture) 86482-3-PBS (250385F4). 100 μg. Concentration 1 mg/ml.

Detection antibody: Mouse Ccl17 Recombinant monoclonal antibody, PBS Only (Detector) 86482-2-PBS (250385B8). 100 μg. Concentration 1 mg/ml.

Matched pair protein standard: Eg3319

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 86482-3-PBS
Tested Reactivity mouse
Immunogen Ccl17 fusion protein Eg3319
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name chemokine (C-C motif) ligand 17
Synonyms
GenBank accession numberNM_011332.3
Gene symbol Ccl17
UniprotF6R5P4
Gene ID (NCBI) 20295
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 86482-2-PBS
Tested Reactivity mouse
Immunogen Ccl17 fusion protein Eg3319
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name chemokine (C-C motif) ligand 17
Synonyms
GenBank accession numberNM_011332.3
Gene symbol Ccl17
UniprotF6R5P4
Gene ID (NCBI) 20295
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
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