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Anti-Human CD20 Mouse scFv Antibody (SF65575-1-MR)

Anti-Human CD20 Mouse scFv Antibody

CD20 Recombinant Antibody for FC

Cat No. SF65575-1-MR

Clone No. 2H7

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Host / Type

Mouse / scFv

Reactivity

human, cynomolgus monkey

Applications

Synonyms

2H7, B lymphocyte antigen CD20, B1, B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1

Formulation: HEPES, EDTA, CHAPS, Sucrose, Proclin300

HEPES PBS Only

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 647

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FC experiment of human PBMCs using SF65575-1-MR
1x10^6 human PBMCs were surface stained with 0.25 ug CD20 Recombinant Antibody (SF65575-1-MR, Clone: 2H7) or 0.25 ug Rabbit scFv Isotype Control (SF98136-1-RR, Clone: 240953C9), and PE Anti-6*His, His-Tag Rabbit RecAb (PE-FcA98450, Clone: 251107A1). Cells were then stained with CoraLite® Plus 647 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of human PBMCs using SF65575-1-MR
1x10^6 human PBMCs were surface stained with 0.25 ug CD20 Recombinant Antibody (SF65575-1-MR, Clone: 2H7) (red) or 0.25 ug Rabbit scFv Isotype Control (SF98136-1-RR, Clone: 240953C9) (blue), and PE Anti-6*His, His-Tag Rabbit RecAb (PE-FcA98450, Clone: 251107A1). Cells were not fixed. Lymphocytes were gated.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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What's the best dilution for CD20 in ?

Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive FC detected in

human PBMCs

Recommended dilution

Application	Dilution
Flow Cytometry (FC)	FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

SF65575-1-MR targets CD20 in FC applications and shows reactivity with human, cynomolgus monkey samples.

Tested Reactivity	human, cynomolgus monkey
Host / Type	Mouse / scFv
Class	Recombinant
Type	Single-chain variable fragment antibody (scFv)
Immunogen	Human tonsillar B cells Predict reactive species
Full Name	membrane-spanning 4-domains, subfamily A, member 1
Calculated Molecular Weight	297 aa, 33 kDa
GenBank Accession Number	BC002807
Gene Symbol	CD20
Gene ID (NCBI)	931
RRID	AB_3750140
Conjugate	Unconjugated
Source	Anti-Human CD20 scFv from clone 2H7, consisting of VH-GGGGS linker-VL (N- to C-terminus), with a His-C tag at the C-terminus.
Form	Liquid
Purification Method	Affinity purification
Storage Buffer	50mM HEPES, 150mM NaCl, 5mM EDTA, 0.1% CHAPS, 10% Sucrose and 0.1% Proclin300, pH 7.3.
Storage Conditions	Store at 2-8°C. Stable for one year after shipment. 20ul sizes contain 0.1% BSA.

Background Information

CD20 is a 33-37 kDa transmembrane phosphoprotein belonging to the membrane-spanning 4A family (PMID: 3260267; 16785532). CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells (PMID: 7524522). CD20 functions as calcium-permeable cation channel (PMID: 7684739). It is involved in the regulation of B-cell activation, proliferation and differentiation (PMID: 7524522).