Anti-Human CD20 Mouse scFv Antibody

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• Anti-Human CD20 Mouse scFv Antibody (SF65575-1-MR)

CD20 Recombinant Antibody for FC

Cat No. SF65575-1-MR Copied!

Clone No. 2H7

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Host / Type

Mouse / scFv

Reactivity

human, cynomolgus monkey

Applications

FC

Synonyms

2H7, B lymphocyte antigen CD20, B1, B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1

• 

  FC experiment of human PBMCs using SF65575-1-MR

  1x10^6 human PBMCs were surface stained with 0.25 ug CD20 Recombinant Antibody (SF65575-1-MR, Clone: 2H7) or 0.25 ug Rabbit scFv Isotype Control (SF98136-1-RR, Clone: 240953C9), and PE Anti-6*His, His-Tag Rabbit RecAb (PE-FcA98450, Clone: 251107A1). Cells were then stained with CoraLite® Plus 647 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of human PBMCs using SF65575-1-MR

  1x10^6 human PBMCs were surface stained with 0.25 ug CD20 Recombinant Antibody (SF65575-1-MR, Clone: 2H7) (red) or 0.25 ug Rabbit scFv Isotype Control (SF98136-1-RR, Clone: 240953C9) (blue), and PE Anti-6*His, His-Tag Rabbit RecAb (PE-FcA98450, Clone: 251107A1). Cells were not fixed. Lymphocytes were gated.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

SF65575-1-MR targets CD20 in FC applications and shows reactivity with human, cynomolgus monkey samples.

• Tested Reactivity: human, cynomolgus monkey
• Host / Type: Mouse / scFv
• Class: Recombinant
• Type: Single-chain variable fragment antibody (scFv)
• Immunogen:

  Human tonsillar B cells

• Full Name: membrane-spanning 4-domains, subfamily A, member 1
• Calculated Molecular Weight: 297 aa, 33 kDa
• GenBank Accession Number: BC002807
• Gene Symbol: CD20
• Gene ID (NCBI): 931
• RRID: AB_3750140
• Conjugate: Unconjugated
• Source: Anti-Human CD20 scFv from clone 2H7, consisting of VH-GGGGS linker-VL (N- to C-terminus), with a His-C tag at the C-terminus.
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: 50mM HEPES, 150mM NaCl, 5mM EDTA, 0.1% CHAPS, 10% Sucrose and 0.1% Proclin300, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment. 20ul sizes contain 0.1% BSA.

Background Information

CD20 is a 33-37 kDa transmembrane phosphoprotein belonging to the membrane-spanning 4A family (PMID: 3260267; 16785532). CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells (PMID: 7524522). CD20 functions as calcium-permeable cation channel (PMID: 7684739). It is involved in the regulation of B-cell activation, proliferation and differentiation (PMID: 7524522).