Tested Applications
| Positive WB detected in | HepG2 cells, Jurkat cells, HeLa cells |
| Positive IHC detected in | human liver cancer tissue, human breast cancer tissue, human hepatocirrhosis tissue, human liver tissue, human normal colon, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human breast cancer tissue, human kidney tissue |
| Positive IF-Fro detected in | mouse brain tissue |
| Positive IF/ICC detected in | HepG2 cells, hiPSC-derived cortical astrocytes (7 DIV) |
| Positive FC (Intra) detected in | HeLa cells, mMSCs cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunohistochemistry (IHC) | IHC : 1:2500-1:10000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10366-1-AP targets Vimentin in WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, CoIP, RIP, IP-MS, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, rabbit, monkey, chicken, goat, sheep, deer |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0489 Product name: Recombinant human Vimentin protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-466 aa of BC000163 Sequence: MSTRSVSSSSYRRMFGGPGTASRPSSSRSYVTTSTRTYSLGSALRPSTSRSLYASSPGGVYATRSSAVRLRSSVPGVRLLQDSVDFSLADAINTEFKNTRTNEKVELQELNDRFANYIDKVRFLEQQNKILLAELEQLKGQGKSRLGDLYEEEMRELRRQVDQLTNDKARVEVERDNLAEDIMRLREKLQEEMLQREEAENTLQSFRQDVDNASLARLDLERKVESLQEEIAFLKKLHEEEIQELQAQIQEQHVQIDVDVSKPDLTAALRDVRQQYESVAAKNLQEAEEWYKSKFADLSEAANRNNDALRQAKQESTEYRRQVQSLTCEVDALKGTNESLERQMREMEENFAVEAANYQDTIGRLQDEIQNMKEEMARHLREYQDLLNVKMALDIEIATYRKLLEGEESRISLPLPNFSSLNLRETNLDSLPLVDTHSKRTLLIKTVETRDGQVINETSQHHDDLE Predict reactive species |
| Full Name | vimentin |
| Calculated Molecular Weight | 466 aa, 54 kDa |
| Observed Molecular Weight | 54 kDa |
| GenBank Accession Number | BC000163 |
| Gene Symbol | VIM |
| Gene ID (NCBI) | 7431 |
| ENSEMBL Gene ID | ENSG00000026025 |
| RRID | AB_2273020 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P08670 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level. Isoforms of vimentin (49 kDa and 60 kDa) had also been reported. (PMID: 8640945, 22728585).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Vimentin antibody 10366-1-AP | Download protocol |
| IF protocol for Vimentin antibody 10366-1-AP | Download protocol |
| IHC protocol for Vimentin antibody 10366-1-AP | Download protocol |
| WB protocol for Vimentin antibody 10366-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Vimentin Polyclonal antibody (Cat# 10366-1-AP) has accumulated 2,614 citations. These publications appear in high-impact journals including Nature, Cell, Nature Communications, Molecular Cancer, Cell Death & Disease, Theranostics, Journal of Experimental & Clinical Cancer Research, Advanced Science, Cancer Research, and Cell Research. Research fields span oncology (cancer metastasis, EMT, tumor microenvironment), fibrosis, immune response, stem cell biology, cardiovascular disease, neurodegeneration, drug resistance, and wound healing, reflecting the antibody's broad utility across diverse biological and disease contexts.
| Species | Application | Title |
|---|---|---|
J Hematol Oncol LincK contributes to breast tumorigenesis by promoting proliferation and epithelial-to-mesenchymal transition. | ||
J Hematol Oncol Combine and conquer: manganese synergizing anti-TGF-β/PD-L1 bispecific antibody YM101 to overcome immunotherapy resistance in non-inflamed cancers. | ||
Cell Early rhombic lip Protogenin+ve stem cells in a human-specific neurovascular niche initiate and maintain group 3 medulloblastoma | ||
Mol Cancer circNOX4 activates an inflammatory fibroblast niche to promote tumor growth and metastasis in NSCLC via FAP/IL-6 axis |
Reviews
What customers say
Users consistently rate this Vimentin antibody highly, with most reviews at 5 stars. It performs well in both Western Blot and Immunofluorescence, with reviewers praising strong signal intensity and consistent staining across various tissues and cell lines, including mouse brain, prostate, ovarian cancer, and liver cancer cells. Recommended dilutions range from 1:100 to 1:2500 depending on the application. One reviewer noted slightly inconsistent IF results, and another gave a moderate rating for use in C2C12 cells. Overall, it is regarded as a reliable and high-performing antibody for Vimentin detection.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Qin (Verified Customer) (09-08-2026) | very good antibody for Vimentin WB
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Sama (Verified Customer) (03-26-2026) | Very beautiful staining - very strong staining of vimentin+ ependymal cell processes
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Vernon (Verified Customer) (01-29-2024) | Western blot signal is great with 30 sec exposure secondary antibody 1:5000 1hr. at room temp
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Tobias (Verified Customer) (06-16-2023) | Works well in IF
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Emma (Verified Customer) (02-22-2022) | Works well by IF on FFPE prostate tissue. (Tris-EDTA antigen retrieval).
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Yuki (Verified Customer) (01-07-2022) | Consistently works antibody
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Hala (Verified Customer) (07-08-2021) | Works veryyyy gooddd
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Hala (Verified Customer) (04-11-2021) | very good antibody, works very well
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wei (Verified Customer) (04-27-2020) | I tried in C2C12 cells
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Patricia (Verified Customer) (02-25-2020) | Works well.
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Kishor (Verified Customer) (01-30-2019) | Good results in western blotting but I could not see consistent results in IF
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Jaime (Verified Customer) (01-24-2018) |
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