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What's the best dilution for Osteocalcin/OCN in ?
Tested Applications
| Positive IHC detected in | human osteosarcoma tissue, mouse lung tissue, rat lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | NIH/3T3 cells |
| Positive FC (Intra) detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16157-1-AP targets Osteocalcin/OCN in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | bone gamma-carboxyglutamate (gla) protein |
| Calculated Molecular Weight | 11 kDa |
| GenBank Accession Number | NM_199173 |
| Gene Symbol | Osteocalcin |
| Gene ID (NCBI) | 632 |
| RRID | AB_2878225 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P02818 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Osteocalcin/OCN antibody 16157-1-AP | Download protocol |
| IF protocol for Osteocalcin/OCN antibody 16157-1-AP | Download protocol |
| IHC protocol for Osteocalcin/OCN antibody 16157-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Osteocalcin/OCN Polyclonal antibody (Cat# 16157-1-AP) has 47 citations across journals including Nature Communications, Bioactive Materials, Advanced Science, Acta Biomaterialia, Cell Death & Disease, Materials Today Bio, FASEB Journal, and Bone. Research fields span bone regeneration and osteogenesis, osteoporosis, periodontitis, bone defect repair, osteosarcoma, vascular calcification, diabetic bone loss, and ferroptosis.
| Species | Application | Title |
|---|---|---|
Bioact Mater Intelligent responsive alloy scaffold temporally regulates the immune-osteogenic axis for the treatment of infectious bone defects. | ||
Bioact Mater A mitochondria-targeted nanoantioxidant restores alveolar bone homeostasis in periodontitis by quenching ROS and suppressing the cGAS-STING pathway. | ||
Nat Commun Dnmt1 determines bone length by regulating energy metabolism of growth plate chondrocytes. | ||
Nat Commun Intelligence-responsive wettability switch coating on magnesium implants for treating osteoporotic fracture. | ||
J Nanobiotechnology Piezoelectric heterojunction/polyetherketoneketone composite with enhanced sonodynamic efficacy and nanozyme activities for anti-infection and encouraging neurovascular ossification. | ||
Adv Sci (Weinh) CGRP Enhances the Regeneration of Bone Defects by Regulating Bone Marrow Mesenchymal Stem Cells Through Promoting ANGPTL4 Secretion by Bone Blood Vessels. |
