PPP3CC-specific Polyclonal antibody

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• PPP3CC-specific Polyclonal antibody (55163-1-AP)

PPP3CC-specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 55163-1-AP Copied!

Publications (3)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

PPP3CC, CNA3, CAM-PRP catalytic subunit, CAM PRP catalytic subunit, CALNA3

• 

  WB analysis of fetal human brain using 55163-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 55163-1-AP (PPP3CC-specific antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human brain using 55163-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55163-1-AP (PPP3CC-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human brain using 55163-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55163-1-AP (PPP3CC-specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human brain using 55163-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55163-1-AP (PPP3CC-specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human testis using 55163-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 55163-1-AP (PPP3CC-specific antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach using 55163-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 55163-1-AP (PPP3CC-specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of U2OS using 55163-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using 55163-1-AP (PPP3CC-specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: fetal human brain tissue, human brain tissue, human testis tissue
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55163-1-AP targets PPP3CC-specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: protein phosphatase 3 (formerly 2B), catalytic subunit, gamma isoform
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: NM_005605
• Gene Symbol: PPP3CC
• Gene ID (NCBI): 5533
• RRID: AB_10860906
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P48454
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPP3CC, also named as CALNA3 and CNA3, belongs to the PPP phosphatase family and PP-2B subfamily. It is a calcium-dependent, calmodulin-stimulated protein phosphatase. It may have a role in the calmodulin activation of calcineurin. This antibody is specific to PPP3CC.

Publications

What published studies show

The PPP3CC-specific Polyclonal antibody (Cat# 55163-1-AP) has been cited in 3 publications across three journals: Acta Biomaterialia, BMC Pregnancy and Childbirth, and International Journal of Neuroscience. The associated research fields include stem cell differentiation and cardiovascular biology, obstetric hepatology (intrahepatic cholestasis of pregnancy), and neurology (spinal cord injury biomarker identification). Cited applications include Western blotting and immunohistochemistry, with tested reactivity in human, mouse, and rat samples.

Automatically generated from the 3 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Acta Biomater; Substrate stiffness regulates differentiation of induced pluripotent stem cells into heart valve endothelial cells.; Authors - Hong Cao
rat	IHC	BMC Pregnancy Childbirth; Core biomarkers analysis benefit for diagnosis on human intrahepatic cholestasis of pregnancy; Authors - Yan Fang
mouse	WB	Int J Neurosci; Identification of PANoptosis-related biomarkers in spinal cord injury (SCI) through multi-omics analysis and machine learning.; Authors - Song Xu