4-1BBL/TNFSF9 Recombinant monoclonal antibody

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  WB analysis using 84185-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84185-5-RR (4-1BBL/TNFSF9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of Raji using 84185-5-RR

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using 4-1BBL/TNFSF9 antibody (84185-5-RR, Clone: 241386E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

4-1BBL/TNFSF9 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 84185-5-RR

Clone No.241386E7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

TNFSF9, CD137L, 4-1BB ligand, 4 1BBL, 4 1BB ligand

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Tested Applications

• Positive WB detected in: Daudi cells, Raji cells, K-562 cells, HEK-293T cells
• Positive IF/ICC detected in: Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84185-5-RR targets 4-1BBL/TNFSF9 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Fusion Protein

• Full Name: tumor necrosis factor (ligand) superfamily, member 9
• Calculated Molecular Weight: 254 aa, 27 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC104805
• Gene Symbol: TNFSF9
• Gene ID (NCBI): 8744
• RRID: AB_3671740
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P41273
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TNFSF9, also named as 4-1BBL, belongs to the tumor necrosis factor family. It is a cytokine that binds to TNFRSF9. TNSF9 induces the proliferation of activated peripheral blood T-cells. It may have a role in activation-induced cell death (AICD) and may play a role in cognate interactions between T-cells and B-cells/macrophages.