Tested Applications
| Positive IF/ICC detected in | Raji cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-84185-5 targets 4-1BBL/TNFSF9 in IF/ICC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg0010 Product name: Recombinant Human TNFSF9 protein (His Tag) Source: mammalian cells-derived, pHZ-KIsec Tag: N-6*his Domain: 71-254 aa of BC104805 Sequence: REGPELSPDDPAGLLDLRQGMFAQLVAQNVLLIDGPLSWYSDPGLAGVSLTGGLSYKEDTKELVVAKAGVYYVFFQLELRRVVAGEGSGSVSLALHLQPLRSAAGAAALALTVDLPPASSEARNSAFGFQGRLLHLSAGQRLGVHLHTEARARHAWQLTQGATVLGLFRVTPEIPAGLPSPRSE Predict reactive species |
| Full Name | tumor necrosis factor (ligand) superfamily, member 9 |
| Calculated Molecular Weight | 254 aa, 27 kDa |
| Observed Molecular Weight | 35 kDa |
| GenBank Accession Number | BC104805 |
| Gene Symbol | TNFSF9 |
| Gene ID (NCBI) | 8744 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P41273 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TNFSF9, also named as 4-1BBL, belongs to the tumor necrosis factor family. It is a cytokine that binds to TNFRSF9. TNSF9 induces the proliferation of activated peripheral blood T-cells. It may have a role in activation-induced cell death (AICD) and may play a role in cognate interactions between T-cells and B-cells/macrophages.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 4-1BBL/TNFSF9 antibody CL488-84185-5 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |

