5 Lipoxygenase Monoclonal antibody, PBS Only

5 Lipoxygenase Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3B1B7

Cat no : 66326-1-PBS

Synonyms

5 lipoxygenase, 5 LO, 5 LOX, 5LPG, ALOX5, arachidonate 5 lipoxygenase, LOG5

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Validation Data Gallery

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  WB analysis of pig spleen using 66326-1-Ig (same clone as 66326-1-PBS)

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 66326-1-Ig (5 Lipoxygenase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66326-1-PBS in a different storage buffer formulation.

• 

  WB analysis of THP-1 using 66326-1-Ig (same clone as 66326-1-PBS)

  WB result of 5 Lipoxygenase antibody (66326-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-5 Lipoxygenase transfected THP-1 cells. This data was developed using the same antibody clone with 66326-1-PBS in a different storage buffer formulation.

• 

  WB analysis of THP-1 using 66326-1-Ig (same clone as 66326-1-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 66326-1-Ig (5 Lipoxygenase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66326-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig spleen using 66326-1-Ig (same clone as 66326-1-PBS)

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 66326-1-Ig (ALOX5 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66326-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 66326-1-Ig (same clone as 66326-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66326-1-Ig (ALOX5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66326-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 66326-1-Ig (same clone as 66326-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66326-1-Ig (ALOX5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66326-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 66326-1-Ig (same clone as 66326-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66326-1-Ig (ALOX5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66326-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 66326-1-Ig (same clone as 66326-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66326-1-Ig (ALOX5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66326-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 66326-1-Ig (same clone as 66326-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66326-1-Ig (ALOX5 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66326-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 66326-1-Ig (same clone as 66326-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66326-1-Ig (ALOX5 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66326-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66326-1-PBS targets 5 Lipoxygenase in WB, IHC, ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: 5 Lipoxygenase fusion protein Ag18168
• Full Name: arachidonate 5-lipoxygenase
• Calculated Molecular Weight: 674 aa, 78 kDa
• Observed Molecular Weight: 78 kDa
• GenBank Accession Number: BC130332
• Gene Symbol: 5 Lipoxygenase
• Gene ID (NCBI): 240
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

ALOX5(arachidonate 5-lipoxygenase) is also named as LOG5, 5-LO and belongs to the lipoxygenase family. It catalyzes the first step in leukotriene biosynthesis, and plays a role in inflammatory processes. It is a 78 kDa iron-containing enzyme that translocates from the cytosol to associate with FLAP, and converts arachidonate in two steps into the unstable intermediate LTA4. It is essential for cysteinyl-leukotriene (cys-LT) production, critical mediators in asthma(PMID:12911785).