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5 Lipoxygenase Monoclonal antibody

5 Lipoxygenase Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a


human, pig







Cat no : 66326-1-Ig


5 lipoxygenase, 5 LO, 5 LOX, 5LPG, ALOX5, arachidonate 5 lipoxygenase, LOG5

Tested Applications

Positive WB detected inpig spleen tissue, THP-1 cells
Positive IHC detected inhuman lung tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66326-1-Ig targets 5 Lipoxygenase in WB, IF, IHC, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen 5 Lipoxygenase fusion protein Ag18168
Full Name arachidonate 5-lipoxygenase
Calculated Molecular Weight 674 aa, 78 kDa
Observed Molecular Weight 70-78 kDa
GenBank Accession NumberBC130332
Gene Symbol 5 Lipoxygenase
Gene ID (NCBI) 240
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ALOX5(arachidonate 5-lipoxygenase) is also named as LOG5, 5-LO and belongs to the lipoxygenase family. It catalyzes the first step in leukotriene biosynthesis, and plays a role in inflammatory processes. It is a 78 kDa iron-containing enzyme that translocates from the cytosol to associate with FLAP, and converts arachidonate in two steps into the unstable intermediate LTA4. It is essential for cysteinyl-leukotriene (cys-LT) production, critical mediators in asthma(PMID:12911785).


Product Specific Protocols
WB protocol for 5 Lipoxygenase antibody 66326-1-IgDownload protocol
IHC protocol for 5 Lipoxygenase antibody 66326-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cancer Gene Ther

Knock-out of 5-lipoxygenase in overexpressing tumor cells-consequences on gene expression and cellular function

Authors - Hannah Weisser

Life Sci Alliance

Three-dimensional growth reveals fine-tuning of 5-lipoxygenase by proliferative pathways in cancer

Authors - Tamara Göbel

Kaohsiung J Med Sci

Magnolin alleviated DSS-induced colitis by inhibiting ALOX5-mediated ferroptosis

Authors - Ting Yao

Acta Diabetol

Identification of potential key lipid metabolism-related genes involved in tubular injury in diabetic kidney disease by bioinformatics analysis

Authors - Yuanshuo Fan