Validation Data Gallery
|Positive WB detected in
|pig spleen tissue, THP-1 cells
|Positive IHC detected in
|human lung tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)
|WB : 1:1000-1:4000
|IHC : 1:100-1:400
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
66326-1-Ig targets 5 Lipoxygenase in WB, IHC, IF, ELISA applications and shows reactivity with human, pig samples.
|Host / Isotype
|Mouse / IgG2a
|5 Lipoxygenase fusion protein Ag18168
|Calculated molecular weight
|674 aa, 78 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Protein A purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
ALOX5(arachidonate 5-lipoxygenase) is also named as LOG5, 5-LO and belongs to the lipoxygenase family. It catalyzes the first step in leukotriene biosynthesis, and plays a role in inflammatory processes. It is a 78 kDa iron-containing enzyme that translocates from the cytosol to associate with FLAP, and converts arachidonate in two steps into the unstable intermediate LTA4. It is essential for cysteinyl-leukotriene (cys-LT) production, critical mediators in asthma(PMID:12911785).
Cancer Gene Ther
Knock-out of 5-lipoxygenase in overexpressing tumor cells-consequences on gene expression and cellular function
Life Sci Alliance
Three-dimensional growth reveals fine-tuning of 5-lipoxygenase by proliferative pathways in cancer