5 Lipoxygenase Monoclonal antibody

5 Lipoxygenase Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

3B1B7

Cat no : 66326-1-Ig

Synonyms

5 lipoxygenase, 5 LO, 5 LOX, 5LPG, ALOX5, arachidonate 5 lipoxygenase, LOG5

---

Validation Data Gallery

• 

  WB analysis of pig spleen using 66326-1-Ig

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 66326-1-Ig (5 Lipoxygenase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of THP-1 using 66326-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 66326-1-Ig (5 Lipoxygenase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig spleen using 66326-1-Ig

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 66326-1-Ig (ALOX5 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung using 66326-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66326-1-Ig (ALOX5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 66326-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66326-1-Ig (ALOX5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 66326-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66326-1-Ig (ALOX5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 66326-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66326-1-Ig (ALOX5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 66326-1-Ig

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66326-1-Ig (ALOX5 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human spleen using 66326-1-Ig

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66326-1-Ig (ALOX5 Antibody) at dilution of 1:200 (under 40x lens).

---

Tested Applications

• Positive WB detected in: pig spleen tissue, THP-1 cells
• Positive IHC detected in: human lung tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:100-1:400

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

66326-1-Ig targets 5 Lipoxygenase in WB, IHC, IF, ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: 5 Lipoxygenase fusion protein Ag18168
• Full Name: arachidonate 5-lipoxygenase
• Calculated molecular weight: 674 aa, 78 kDa
• Observed molecular weight: 78 kDa
• GenBank accession number: BC130332
• Gene symbol: ALOX5
• Gene ID (NCBI): 240
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ALOX5(arachidonate 5-lipoxygenase) is also named as LOG5, 5-LO and belongs to the lipoxygenase family. It catalyzes the first step in leukotriene biosynthesis, and plays a role in inflammatory processes. It is a 78 kDa iron-containing enzyme that translocates from the cytosol to associate with FLAP, and converts arachidonate in two steps into the unstable intermediate LTA4. It is essential for cysteinyl-leukotriene (cys-LT) production, critical mediators in asthma(PMID:12911785).

Publications

Species	Application	Title
human	WB,IF	Cancer Gene Ther; Knock-out of 5-lipoxygenase in overexpressing tumor cells-consequences on gene expression and cellular function; Authors - Hannah Weisser
human	WB,IHC	Life Sci Alliance; Three-dimensional growth reveals fine-tuning of 5-lipoxygenase by proliferative pathways in cancer; Authors - Tamara Göbel