5 Lipoxygenase Monoclonal antibody

5 Lipoxygenase Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig

Applications

WB, IHC

Conjugate

Unconjugated

CloneNo.

3B1B7

Cat no : 66326-1-Ig

Synonyms

5 lipoxygenase, 5 LO, 5 LOX, 5LPG, ALOX5, arachidonate 5 lipoxygenase, LOG5



Tested Applications

Positive WB detected inpig spleen tissue
Positive IHC detected inhuman lung tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Sample-dependent, check data in validation data gallery

Product Information

66326-1-Ig targets 5 Lipoxygenase in WB, IHC applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen 5 Lipoxygenase fusion protein Ag18168
Full Name arachidonate 5-lipoxygenase
Calculated molecular weight 674 aa, 78 kDa
Observed molecular weight 78 kDa
GenBank accession numberBC130332
Gene symbol ALOX5
Gene ID (NCBI) 240
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ALOX5(arachidonate 5-lipoxygenase) is also named as LOG5, 5-LO and belongs to the lipoxygenase family. It catalyzes the first step in leukotriene biosynthesis, and plays a role in inflammatory processes. It is a 78 kDa iron-containing enzyme that translocates from the cytosol to associate with FLAP, and converts arachidonate in two steps into the unstable intermediate LTA4. It is essential for cysteinyl-leukotriene (cys-LT) production, critical mediators in asthma(PMID:12911785).

Protocols

Product Specific Protocols
WB protocol for 5 Lipoxygenase antibody 66326-1-IgDownload protocol
IHC protocol for 5 Lipoxygenase antibody 66326-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols